Genetic Diversity Analysis of Wheat Rhizobacterial Isolates from North Indian Soils Showed the Unique Occurrence of Sporosarcina Related Species

Abstract

In natural ecosystem an intimate relationship between staggering bacterial diversity and plants are observed and such a relationship can be friendly or hostile. Soil being a base of plant root – microbe interaction, serves as an excellent source of research at genetic levelowing to new techniques related to sequencing and metagenomics. The microbial diversity within rhizosphere of wheat plant from agricultural soils of north India were characterized by direct soil DNA extraction, restrictinon fragment length polymorphism (RFLP) pattern analysis of 16S gene and subsequent sequencing of V6-V9 region of 16S rRNA for clones having unique restriction patterns. Analysis of wheat rhizosphere from different regions of North India showed the presence of rich microbial diversity. Both gram positive and Gram negative bacterial communities, reported to be promoting growth activity, were found to be present in all the regions of wheat cultivation. Most of the bacteria belonged to phylum proteobacteria and firmicutes, uncultured bacterium belonging to unknown phylogeny was also found to be present. Although no regional variation was found, the occurrence of Sporosarcina related species were unique to wheat rhizosphere.