PCR Identification of Urinary Pseudomonas aeruginosa from Pregnant Women Attending a Tertiary Health Care Centre in Makurdi, Central Nigeria

Abstract

In this study 28 isolates of P. aeruginosa were obtained from the urine of pregnant women attending antenatal in a tertiary hospital in Makurdi, Central Nigeria. Urinary tract infection is one of the most frequently seen ‘medical' complications in pregnancy.P. aeruginosa was isolated from (10.1) of urine samples. But using PCR- based amplification of the specific P. aeruginosaprimers proved a 100% specificity.Several results indicated that preliminary phenotypic testing had misidentified several isolates. The 16S rDNA sequence was determined for 28 isolates, and in all cases it confirmed the results of the PCR assays.