Optimization of Various Parameters Used in Immobilizing Acremonium Chrysogenum 1391 for Cephalosporinproduction

Abstract

The present study deals with the examination of cephalosporin production by Acremonium chrysogenum 1391 would be improved with sodium alginate immobilization in submerged fermentation when compared with free cells. Results showed that in free cells produced 145 to 150µg/mL of cephalosporin whereas immobilized cells produced 360-380 µg/mL. Immobilization of cells retards the growth rate of Acremonium chrysogenum but increased the length of growth period and improved the cephalosporin production. The optimum immobilized conditions were alginate 3%, Inoculum size 3mL, the culture temperature at 28°C and bead diameter 2.50mm. Cephalosporin production increased with increasing Inoculum density, but decreased with increasing bead diameter.