Production and Optimization of Extra-Cellular Enzymes of Actinobacteria Isolated from Textiles Dye Polluted Soils of Tirupur, South India

Abstract

The diversity and the distribution of actinobacteria and their antimicrobial activities were studied and reported in the textile dye polluted soil of Tirupur (Dollar City of Tamilnadu), Xouth India. Physico-chemical properties of the soil samples were analyzed. As much as twenty actinobacterial isolates were isolated from ten different soil samples. They were characterized and grouped into 8 genera viz. Streptomyces (5), Pseudonocardia (4), Actinopolyspora (3), Actinoplanes (2), Nocardia (2) Nocardiopsis (2), Streptoalloteichus (2) and Actinomyces (1). All the twenty isolates were screened for their extra-cellular enzyme activity including amylase, cellulase, chitinase, L-asparaginase, keratinase and proteasae. Among the 20 isolates, maximum of 7 isolates had L-asparaginase activity, followed by 6 isolates with chitinase activity, 3 isolates with amylase activity, 2 isolates with protease activity, 2 isolates with cellulase activity, 2 isolates with keratinase activity, while seven isolates did not produce any enzyme. Actinobacteria with strong enzyme activity were characterized based on the morphological, biochemical, cultural and physiological properties. Furthermore, the growth and enzyme productivity of the isolates were optimized with respect to following parameters such as pH, temperature, salinity, media, carbon and nitrogen sources.