Isolation & Biochemical Characterization of Halotolerant Plant Growth Promoting Rhizobacteria

Abstract

Rhizobacteria possessing multiple plant growth promoting activities. Halotolerant rhizobacteria were isolated from the groundnut soil sample with salinity 0.7mS/cm in Jodiya region of saurashtra. 48 bacterial isolates were obtained among which 45 isolates showed halotolerancy upto 12% NaCl concentration and different tests were performed to find plant growth promoting traits at 2% NaCl concentration.  like NITROGEN FIXATION by bacteria in which all the bacteria has shown us that capacity. By performing the PHOSPHATE SOLUBILIZING TEST  24 isolates were positive. AMMONIA TEST and in IAA PRODUCTION TEST all isolates had given positive results and the maximum indole production was 62.18µg/ml. And in ANTIFUNGAL TEST 4 isolates had given positive result. Further several qualitative enzymatic test are performed like protease, catalase, amylase, cellulase, lipase. After that biochemical tests like MR test, VP test, H2S production test, H₂O₂ test, nitrate reduction test, TSI agar test, urea hydrolysis, citrate utilization test were peformed.