Structural Characterization of Glycolipid Synthesized from Chlorella Pyrenoidosa NCIM 2738 by FT-IR and GC-MS and Its Effect on Fungal Spore Germination

Abstract

Unicellular microalgae Chlorella pyrenoidosa NCIM 2738 was able to produce glycolipid biosurfactant. Chemical nature of biosurfactant analyzed by Fourier transform infrared spectroscopy showed presence of functional groups for glycolipid was determined by peaks found at 1708 cm^-1 confirmed for stretching of C=O primarily from lipids and at 1260-968 cm^-1 was for C–O–C stretching of carbohydrates. Gas chromatography-mass spectroscopy analysis was confirmed after fatty acid and sugar analysis where the hydrophobic moiety of glycolipid envisaged being a C16 methyl ester and presence of carbohydrates glucose and galactose after alditol acetate derivatization. Application of glycolipid as antifungal agent was determined after the inhibition of fungal spore germination of Aspergillus niger NCIM 545.