Determination of Antitrypsin Activity of Lupeol by Folin Lowery Method

Abstract

Lupeol is a compound which found in most of the plants such as Crataeva nurvala. Lupeol was isolated from the stem bark powder of Crataeva nurvala by Soxhlet extraction in 95% ethanol, and then identified by Thin Layer Chromatography, violet colored spot was observed on TLC plates which confirmed the presence of Lupeol. Libbermman Burchard test which is a biochemical test was done for further confirmation of Lupeol in extract. Antitrypsin (Anti-protease) activity was determined by using Folin lowery method which is generally used for protein assay.