Development of an Electrochemical Microsystin-LR Aptasensor Using Iron Nanoparticles Synthesized from Ripe Banana Peels Extract

Abstract

In this work, an electrochemical aptasensor was developed for the detection of Microcystin-leucine arginine (MC-LR) by immobilizing MC-LR targeting aptamers onto electro-deposited polyaniline (PANI) doped with synthesised iron nanoparticles (FeNPs). Aptamer microcystin binding event was monitored and recorded by cyclic voltammetry. The linear range (LR) and the limit of detection (LOD) of the aptasensor were from 0.0 – 1.0 µg L^-1and 0.06 µgL^-1 respectively. The World Health Organization (WHO) has set a concentration limit of 1µgL^-1for MC-LR in drinking water, indicating that the developed aptasensor was enough for practical application. Therefore, this electrochemical aptasensor can be applied for detection of microcystin-LR in drinking water samples.