A Preliminary Biochemical Screening of Spermacoce Hispida Linn Seeds in Fenton Induced Toxicity in Chicken Smooth Muscle

Abstract

Spermacoce hispida (family; Rubiaceae) is widely used as an Ayurvedic folk medicine. The present study is focused on preliminary phytochemical and cytoprotective effect of Spermacoce hispida and was investigated against Fenton mixture induced oxidative stress in isolated chicken ileum preparation. The seed of Spermacoce hispida was extracted with different organic solvents in increasing order of polarity. The preliminary phytochemical investigations were determined using hydroalcoholic extract of Spermacoce hispida. In biochemical- pharmacological activity, we evaluate the effect of hydro alcoholic extract of Spermacoce hispida in oxidative stressed ileum preparation invitro. Group I tissues received vehicle. Group II tissues were oxidatively stressed with Fenton mixture. Group III and IV tissues were treated with two graded doses of naraginine after the administration of Fenton mixture. Group V, VI and VII tissues were superfused with three graded doses of hydro alcoholic extract of Spermacoce hispida with Fenton mixture. The toxicity of Fenton mixture was objectified by significant difference of malondialdehyde (TBARS), Reduced glutathione (GSH), Glutathione peroxidase (GPx) and catalase, (p<0.05) in oxidative stressed tissues. On preconditioned tissues with extract (10, 20 and 40mg), the oxidative stress was decreased with increase in antioxidants level. This effect of extract was found to be dose dependent. Based on the present findings Fenton mixture induced toxicity in isolated superfused tissues was significantly antagonized by the antioxidant activity of Spermacoce hispida extract.