Chromatographic Analysis of Free Amino Acid of Acrida Luqubris A. Giqantia and Chrotogonus Trachypterus

Abstract

of three species of grasshoppers viz. C. trachypterus, A.gigantea and A. lugubris has been made with the view to find out the qualitative and quantitative differences in them. The concentration of the FAA arginine, dihydroxyphenylalanine, DL-alanine, isoleucine, proline, hydroxyproline has been found to be much higher in the nymphs as compared to both the adults males and females of C.trachypterus. Tyrosine and Nor-leucine have been found to be absent in the males but seem to be present in the females of C. trachypterus. However, the 2 amino acids lysine and methionine present in the males could not be detected in the females of this species.  An unidentified spot was observed in the females of C.  trachypterus. Further, the concentration of FAA glycine, DL-alanine and isoleucine were found to be higher in the females than the males. The concentration of FAA arginine, dihydroxyphenylalanine, glycine and tryptophan to be highr in the females than the males of A.gigantea. Lysine and methionine identified in the males could not be detected in the females. A yellow coloured band has been noticed only in the males but no free amino acid could be identified in it. The two sexes of A.lugubris have also revealed the differences in their FAA pattern .The amino acids histidine, aspartic acid glutamic acid and threonine have been found only in the females whereas the three amino acids lysine, methionine and butyric acid could be detected only in the males of this species. The concentration of FAA arginine, phenylalanine and valine was seen to be higher in the females as compared to the males. The comparison of the FAA pattern in the adults of three species of grasshoppers viz. C.trachypterus, A.gigantea and A.lugubris has shown differences not only in the number and type of FAA in them but also in their concentration. The FAA of the various body parts the adult females of A. lugubris. The amino acids lysine, methionine, butyric acid, tyrosine and nor-leucine detected in the thorax were found to be absent in the head and abdomen. It is the glutamic acid which is absent in the thorax but is present in head and abdomen. The maximum concentration of FAA, arginine, dihydroxyphenylalanine, Dl-alanine and isoleucine detected in the thorax region only.