Preparation of Lyophilized Anti-Rabies Immunoglobulin

Abstract

This work aims to provide anti-rabies IgG in a purified concentrated dose smaller than that of the liquid anti-rabies whole serum. Such purpose was fulfilled through lyophilization of anti-rabies IgG precipitated from rabies hyper immune serum prepared in horse. Such serum was found to have an antibodies titer of 105IU/ml with immunoglobulin value of 4.3g/dl. Testing of the obtained final product revealed that it was free from foreign contaminants, safe in mice and able to stop rabies infection in experimentally infected mice. It was found that administration of IgG in mice experimentally infected with rabies virus at concentrations of 4.30, 2.20, 1.10, 0.54, 0.27 and 0.14g/dl showed protection percentage of 100; 90; 80; 80; 70 and 60% respectively when such treatment was applied from the zero time to the 3^rd day post exposure. Latent treatment leads to progression of the infection where the protection rate decreased to be 40, 30, 30,20,and 10% and when administration of IgG was carried out on the 4^th day post exposure to record 0% by the 6^th day. Depending on these findings, it could be concluded that the lyophilized anti-rabies IgG is safe and able to withstand the virus infection when administrated on the correct time with the suitable dose.