Invitroshoot Multiplication Response from Shoot Tips of BergenialigulataEngl. On Different Nutrient Media- A Comparative Study

Abstract

Invitro raised shoot tips of Bergenialigulata were cultured on various media viz. MS (both full and half strength), modified MS, Gamborg's, Nitsch & Nitsch and White's media to score better shoot multiplication response for effective micropropagation. All these media were supplemented with varying concentration of Kinetin (2.5-15μM) and same concentration of IAA i.e. 7.5μM. Although very little shoot regenerations was observed on MS full strength, Gamborg's, and Nitsch & Nitsch media, modified MS medium was found to be best medium for shoot regeneration, supplemented with Kinetin and IAA. MS half strength and White's media did not show any response in terms of shoot formation.