Activity and Stability of Linamarase from Cassava (Manihot Esculenta Crantz) Leaves and its Ability to Hydrolyze Linamarin

Abstract

Linamarase from cassava leaves was studied its activity, stability, and ability to degrade linamarin.  Linamarase was extracted from young cassava leaves using 0.2 M phosphate buffer pH 6.0. Linamarin as a substrate for linamarase was extracted from young cassava leaves using 0.1 M hydrogen chloride (HCl). The activity of crude linamarase was examined at various pH (4.0 to 7.0), and temperatures (30 to 75°C). Stability of crude linamarase at various temperatures was conducted at 40 to 70°C for 240 minutes. The activity of crude linamarase was 306.13mU/mL. Yields of linamarase and linamarin were 244.08mU/g leaf and 338.50µg hydrogen cyanide (HCN) eq./g leaf respectively. The enzyme showed the highest activity at pH 5.5 and temperature 55°C. Crude linamarase showed good stability at temperature of 40, 50, and 60°C for 240 minutes with the remaining activity more than 90%. Hydrolysis of linamarin using crude linamarase resulted in the release of more than 90% HCN within 30 minutes incubation. Linamarase extracted from young leaves cassava has potent  as an exogenous enzyme to hydrolyze linamarin from cassava.