The Effect of Inducers, Nitrogen Compound and Glucose on the Uricase Production by Lactobacillus plantarum Dad-13

Abstract

Uricase plays an important role in the reducing of serum uric acid in human and can be used as a diagnostic reagent. This research aimed to evaluate the effect of inducers, nitrogen and glucose in the medium on intracellular uricase production by Lactobacillus plantarum Dad-13.  Pepton Glucose Yeast Extract (PGY) with or without glucose and pepton was used as a medium for uricase production while adenine, guanine and uric acid were added into the medium as an inducer. The result showed that uricase produced by L. plantarum Dad-13 is an inducible enzyme which needs uric acid as an inducer while adenine and guanine can not induce uricase production. Glucose and peptone play an important role on uricase production by L. plantarum Dad-13 while peptone is needed for uric acid transportation into the cell. The highest of uricase production resulted from PGY medium and the lowest resulted from PGY medium without peptone supplementation due to lower intracellular of uric acid.